The Atlantic Coast Line Railroad's Lakeland—Fort Myers Line was a rail line in Central and Southwest Florida. It ran from Lakeland, Florida south through Bartow, Arcadia, and Punta Gorda to Fort Myers. It was built incrementally from 1884 to 1926 and at its greatest extent, it ran as far south as Marco Island. Even though it was essentially a continuous line, the segment south of Fort Myers was classified separately on employee timetables as the Fort Myers Southern Branch.