The Nesttun–Os Line (Norwegian: Nesttun–Osbanen) was a narrow gauge railway between Nesttun, now part of Bergen, and the community of Osøyro in Os municipality, Norway. As the first private railway in Norway, it opened 1 June 1894, designed to connect Os to the Voss Line, allowing for passenger and freight transport to Bergen and Voss. Despite a boom caused by World War I, the railway was eventually driven out of business by competition from road transport, which provided faster service. On 2 September 1935, it became the first Norwegian railway to close, and most of the railway was dismantled the following year.