Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products
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| - Nucleasa S1 de Aspergillus (es)
- Nuclease S1 (en)
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| - La nucleasa S1 de Aspergillus (EC 3.1.30.1) es una enzima endonucleasa extraída de Aspergillus oryzae que rompe moléculas de ADN de cadena simple (ADNcs) y ARN para formar oligo o mononucleótidos. Esta enzima cataliza la siguiente reacción química: Escisión endonucleolítica de ácidos nucleicos de cadena simple para formar 5'-fosfomononucleótidos y 5'-fosfooligonucleótidos (es)
- Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products (en)
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| name
| - Nuclease S1 (en)
- Nuclease P1 (en)
- S1-P1 nuclease (en)
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| - P1 nuclease in complex with a substrate analog (en)
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| Organism
| - Aspergillus oryzae (en)
- Penicillium citrinum (en)
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| - NuP1 (en)
- NucS (en)
- S1-P1_nuclease (en)
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| - La nucleasa S1 de Aspergillus (EC 3.1.30.1) es una enzima endonucleasa extraída de Aspergillus oryzae que rompe moléculas de ADN de cadena simple (ADNcs) y ARN para formar oligo o mononucleótidos. Esta enzima cataliza la siguiente reacción química: Escisión endonucleolítica de ácidos nucleicos de cadena simple para formar 5'-fosfomononucleótidos y 5'-fosfooligonucleótidos Aunque la enzima tiene preferencia por los sustratos de cadena simple, ocasionalmente puede introducir rupturas en moléculas de ADN o ARN de doble cadena, o en híbridos ADN-ARN. La enzima hidroliza las regiones de cadena simple que se forman en los bucles o aperturas del ADN dúplex. (es)
- Nuclease S1 (EC 3.1.30.1) is an endonuclease enzyme that splits single-stranded DNA (ssDNA) and RNA into oligo- or mononucleotides. This enzyme catalyses the following chemical reaction Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products Although its primary substrate is single-stranded, it can also occasionally introduce single-stranded breaks in double-stranded DNA or RNA, or DNA-RNA hybrids. The enzyme hydrolyses single stranded region in duplex DNA such as loops or gaps. It also cleaves a strand opposite a nick on the complementary strand. It has no sequence specificity. Well-known versions include S1 found in Aspergillus oryzae (yellow koji mold) and Nuclease P1 found in Penicillium citrinum. Members of the S1/P1 family are found in both prokaryotes and eukaryotes and are thought to be associated in programmed cell death and also in tissue differentiation. Furthermore, they are secreted extracellular, that is, outside of the cell. Their function and distinguishing features mean they have potential in being exploited in the field of biotechnology. (en)
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