About: Patch-sequencing

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Patch-sequencing (patch-seq) is a method designed for tackling specific problems involved in characterizing neurons. As neural tissues are one of the most transcriptomically diverse populations of cells, classifying neurons into cell types in order to understand the circuits they form is a major challenge for neuroscientists. Combining classical classification methods with single cell RNA-sequencing post-hoc has proved to be difficult and slow. By combining multiple data modalities such as electrophysiology, sequencing and microscopy, Patch-seq allows for neurons to be characterized in multiple ways simultaneously. It currently suffers from low throughput relative to other sequencing methods mainly due to the manual labor involved in achieving a successful patch-clamp recording on a neuron

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rdfs:label	Patch-sequencing (en)
rdfs:comment	Patch-sequencing (patch-seq) is a method designed for tackling specific problems involved in characterizing neurons. As neural tissues are one of the most transcriptomically diverse populations of cells, classifying neurons into cell types in order to understand the circuits they form is a major challenge for neuroscientists. Combining classical classification methods with single cell RNA-sequencing post-hoc has proved to be difficult and slow. By combining multiple data modalities such as electrophysiology, sequencing and microscopy, Patch-seq allows for neurons to be characterized in multiple ways simultaneously. It currently suffers from low throughput relative to other sequencing methods mainly due to the manual labor involved in achieving a successful patch-clamp recording on a neuron (en)
foaf:depiction
dcterms:subject	Electrophysiology RNA sequencing
Wikipage page ID	66692692 (xsd:integer)
Wikipage revision ID	1095740811 (xsd:integer)
Link from a Wikipage to another Wikipage	Electrophysiology Epigenetics Neural correlates of consciousness Neural network MAP-Seq Methylated DNA immunoprecipitation dbr:Biotin DNA methylation Induced pluripotent stem cell Neurotransmitter Pipette Potassium channel Postsynaptic potential Proteomics Electrophysiology Neuron Ohm Electrical resistance and conductance Conformational change Connectomics Optogenetics Ligand Machine learning Compartmental neuron models Computer vision Dendrite Synapse T-distributed stochastic neighbor embedding Omics Action potential Cell (biology) Transcription (biology) GCaMP Ion channel Feature vector RNA-Seq Cell culture Cell type Flow cytometry Histone acetylation and deacetylation Patch clamp Voltage clamp RNA sequencing Resting potential Threshold potential Autoencoder Automated patch clamp Axon CRISPR Microscopy ChIP sequencing Channelopathy Single cell sequencing Slice preparation Soma (biology) Uniform Manifold Approximation and Projection Voltage-gated calcium channel Voltage-gated ion channel Neuromorphology Neuronal tracing Neurotransmitter receptor Sodium channel List of single cell omics methods Rhodamine Primary visual cortex Resting membrane potential Single-cell RNA-sequencing Multi-electrode array Neuroanatomical atlas
Link from a Wikipage to an external page	https://www.jneurosci.org/content/41/5/937.abstract http://syntheticneurobiology.org/PDFs/19.07.suk.pdf https://www.frontiersin.org/articles/10.3389/fnmol.2018.00363/full
sameAs	Patch-sequencing Patch-sequencing
dbp:wikiPageUsesTemplate	dbt:Reflist
thumbnail	wiki-commons:Special:FilePath/Patch-Seq_Methodology.png?width=300
has abstract	Patch-sequencing (patch-seq) is a method designed for tackling specific problems involved in characterizing neurons. As neural tissues are one of the most transcriptomically diverse populations of cells, classifying neurons into cell types in order to understand the circuits they form is a major challenge for neuroscientists. Combining classical classification methods with single cell RNA-sequencing post-hoc has proved to be difficult and slow. By combining multiple data modalities such as electrophysiology, sequencing and microscopy, Patch-seq allows for neurons to be characterized in multiple ways simultaneously. It currently suffers from low throughput relative to other sequencing methods mainly due to the manual labor involved in achieving a successful patch-clamp recording on a neuron. Investigations are currently underway to automate patch-clamp technology which will improve the throughput of patch-seq as well. (en)
prov:wasDerivedFrom	wikipedia-en:Patch-sequencing?oldid=1095740811&ns=0
page length (characters) of wiki page	54240 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:Patch-sequencing
is Link from a Wikipage to another Wikipage of	Electrophysiology MAP-Seq Patch-Sequencing Patch clamp Automated patch clamp Single cell sequencing

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