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About: Ribosome display     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:PsychologicalFeature100023100, within Data Space : dbpedia.demo.openlinksw.com associated with source document(s)

Type: topical conceptyago:WikicatBiochemistryMethodsyago:Ability105616246yago:Abstraction100002137yago:Cognition100023271yago:Know-how105616786yago:Method105660268yago:PsychologicalFeature100023100 New Facet based on Instances of this Class

Ribosome display is a technique used to perform in vitro protein evolution to create proteins that can bind to a desired ligand. The process results in translated proteins that are associated with their mRNA progenitor which is used, as a complex, to bind to an immobilized ligand in a selection step. The mRNA-protein hybrids that bind well are then reverse transcribed to cDNA and their sequence amplified via PCR. The result is a nucleotide sequence that can be used to create tightly binding proteins.