The Scottish Junior Football Ayrshire Division One (also known as the Ayrshire District League) was the third-tier division of the West Region of the Scottish Junior Football Association. The two highest-placed teams at the end of the season were promoted to the Super League First Division. It sat below two region-wide divisions and alongside a 'Central' geographical equivalent (with two divisions) representing the Ayrshire League and Central League which merged to form the West Region.