U.S. Route 129 (US 129) in Florida is a north–south United States Highway. It runs 88 miles (142 km) from Chiefland north to the Georgia State Line in Levy, Gilchrist, Suwannee, and Hamilton Counties. The secret designation for US 129 in Florida, between Chiefland and Hildreth is SR 49 (SR 49). Between Hildreth and Branford, it follows SR 20, between Branford and Live Oak, it follows SR 249, between Live Oak and Jasper, it follows SR 51, between Hillcoat and Jasper, it follows SR 25, and between Hillcoat and the Georgia border, it follows SR 100. The only signed concurrencies that exist are with US 27 between Hildreth and Branford, with US 41 between Hillcoat and Jasper, and with SR 6 in Jasper.