About: Zoo blot

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A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species.

Attributes	Values
rdf:type	yago:WikicatLaboratoryTechniques yago:Ability105616246 yago:Abstraction100002137 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:PsychologicalFeature100023100 yago:Technique105665146
rdfs:label	Zoo blot (en)
rdfs:comment	A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species. (en)
dct:subject	Animal genetics Molecular biology Laboratory techniques
Wikipage page ID	9059630 (xsd:integer)
Wikipage revision ID	1077625527 (xsd:integer)
Link from a Wikipage to another Wikipage	Animal genetics Nucleic acid hybridization Molecular biology Laboratory techniques Phosphorus DNA sequence Autoradiography Southern blot Fluorescent in situ hybridization
sameAs	Zoo blot Zoo blot Zoo blot Zoo blot
dbp:wikiPageUsesTemplate	dbt:Refimprove dbt:Reflist dbt:Molecular-biology-stub
has abstract	A zoo blot or garden blot is a type of Southern blot that demonstrates the similarity between specific, usually protein-coding, DNA sequences of different species. A zoo blot compares animal species while a garden blot compares plant species. The purpose of the zoo blot is to detect the conservation of the gene(s) of interest throughout the evolution of different species. In order to understand the degree to which a particular gene is similar from species to species, DNA extracts from a set of species are isolated and spread over a surface. Then, a gene probe specific to one of the species is labeled and allowed to hybridize to the prepared DNA. Usually, the probe is marked with a radioactive isotope of phosphorus. Following the hybridization, autoradiography or other imaging techniques are used to identify successfully hybridized probes, proof of similarity between species' genomes. The hybridization between a probe and a segment of DNA will happen even when the strands are similar but not identical. As a result, zoo blotting is used to detect similar or exact relationships between the DNA in question and other organisms. It can also help establish the locations of introns and exons, as the latter will be far more conserved than the former. (en)
gold:hypernym	Blot
prov:wasDerivedFrom	wikipedia-en:Zoo_blot?oldid=1077625527&ns=0
page length (characters) of wiki page	2553 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:Zoo_blot
is Link from a Wikipage to another Wikipage of	Fluorescence in situ hybridization Garden blot
is Wikipage redirect of	Garden blot
is foaf:primaryTopic of	wikipedia-en:Zoo_blot

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