The Ashland Eagles are the athletic teams that represent Ashland University, located in Ashland, Ohio, in Division II intercollegiate sports of the National Collegiate Athletic Association (NCAA). The Eagles are members of the Great Midwest Athletic Conference (G-MAC) for 21 of their 22 varsity sports. They previously had competed in the Great Lakes Intercollegiate Athletic Conference (GLIAC) from 1995–96 to 2020–21, in the Mid-Ohio League (later the American Mideast Conference) of the National Association of Intercollegiate Athletics (NAIA) from 1949–50 to 1965–66, and in the Ohio Athletic Conference (OAC) from 1931–32 to 1947–48.