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Berkeley (/ˈbɑːrkli/ BARK-lee) is a market town and parish in the Stroud District in Gloucestershire, England. It lies in the Vale of Berkeley between the east bank of the River Severn and the M5 motorway. The town is noted for Berkeley Castle, where the imprisoned King Edward II is believed to have been murdered, as well as the birthplace of the physician Edward Jenner, pioneer of the smallpox vaccine, the world's first vaccine. The parish includes the village of Berkeley Heath, which runs along the A38 Bristol to Gloucester road and the adjacent B4066 towards Berkeley.