John Randolph Chambliss Sr. (March 4, 1809 – April 3, 1875) was a Virginia plantation owner and politician who served in the Confederate House of Representatives during the American Civil War. His son, Brigadier General John R. Chambliss Jr., a cavalryman, was killed during the war. After the war, he retired from politics and returned to his home to again practice law. He was interred in the family graveyard near Emporia, Virginia.