No. 16 Operational Training Unit RAF (16 OTU) was a training unit of the Royal Air Force. It was formed at RAF Upper Heyford on 8 April 1940 from the No. 4 Group RAF Pool, which comprised No 7 and No 76 Squadron, within No. 6 Group RAF, to train night bomber crews, for which it was equipped with Handley Page Hampdens and Herefords. Transferred to No. 7 Group RAF on 15 July 1940 until 11 May 1942 when No 7 Group was renumbered No. 92 Group RAF (92 Gp), 16 OTU converted to Vickers Wellington bombers in April 1942 and from March to December 1942 its headquarters moved to RAF Barford St John whilst runways were laid at Upper Heyford, disbanding on 1 January 1945.