Pasquotank County /ˈpæskwətæŋk/ is a county located in the U.S. state of North Carolina. As of the 2020 Census, the population was 40,568. Its county seat is Elizabeth City. The county was originally created as Pasquotank Precinct and gained county status in 1739. Pasquotank County is part of the Elizabeth City, NC Micropolitan Statistical Area, which is also included in the Virginia Beach-Norfolk, VA-NC Combined Statistical Area.