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Prince Charles of Hesse and by Rhine (23 April 1809 - 20 March 1877) was a German prince, officer and politician. He was the second surviving son of Louis II, Grand Duke of Hesse and Wilhelmine of Baden. The prince had a military career and became a general in the infantry of the army of the Grand Duchy of Hesse. He was also a member of the Landtag of Hesse. As his brother Grand Duke Louis III did not have children, he was succeeded by Prince Charles' eldest son, Grand Duke Louis IV.