Thomas Fowler (died 1590) was an English lawyer, diplomat, courtier, spy, servant of the Countess of Lennox, broker of the marriage of Mary, Queen of Scots and Lord Darnley, steward of the Earl of Leicester, advisor to James VI of Scotland and the Scottish ambassador in London, Archibald Douglas. There were two other notable contemporary Thomas Fowlers in London; the "comptroller of the works" (paymaster of the royal works d. 1595), who married for his second wife a Margaret Johnson who has been mistakenly identified as the mother of the poet Ben Jonson; and Sir Thomas Fowler of Islington.