Trout Brook is a tributary of South Branch Tunkhannock Creek in Lackawanna County and Wyoming County, in Pennsylvania, in the United States. It is approximately 5.0 miles (8.0 km) long and flows through West Abington Township in Lackawanna County and Clinton Township in Wyoming County. The stream has no named tributaries, but it does have ten unnamed tributaries. There are also a number of wetlands in the watershed of the stream. The stream is classified as a Coldwater Fishery. Its watershed occupies portions of three municipalities in Wyoming County and five in Lackawanna County.