Twilo was an American nightclub in operation from 1995 to 2001 in New York City and from 2006 to 2007 in Miami. The New York location at 530 West 27th Street in the Chelsea neighborhood of Manhattan regularly attracted a crowd of thousands to its warehouse-like dance floor. Playing host to dozens of DJs from around the world, the club was important in popularizing international styles of house and trance music within the United States.