West Virginia Route 45 (WV 45) is a state highway in the U.S. state of West Virginia. The state highway runs 25.8 miles (41.5 km) from the Virginia state line near Glengary east to WV 230 and WV 480 in Shepherdstown. WV 45 connects the communities of Glengary and Arden in southwestern Berkeley County with the county seat of Martinsburg. The state highway also connects Shepherdstown in northern Jefferson County with Martinsburg, where the highway meets Interstate 81 (I-81), U.S. Route 11 (US 11), and WV 9.