William Barne or Berners (by 1527 – 1562), of Harlton and Milton, Cambridgeshire, was an English landowner and politician. He married Alice Cook, widow of William Cook (d.1553) of Chesterton, Cambridge, who had inherited Milton Manor. He was a Member (MP) of the Parliament of England for Preston, Lancashire, in April 1554. On William's death, his estates passed to his son Robert. Robert, a Roman Catholic convert, fell into financial problems and sold the estates to his brother-in-law, Edward Slegge.