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Statements

Subject Item
dbr:Optical_sectioning
rdf:type
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rdfs:label
Optical sectioning
rdfs:comment
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning.
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dbc:Microscopy
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dbr:Scanning_electron_microscope dbr:Point_spread_function dbr:Detector dbr:Confocal_microscopy dbr:Phase_difference dbr:Microtome dbr:Objective_lens n8:Optical_sectioning_of_pollen.jpg dbr:Oil_immersion dbr:Three_dimensions dbr:Diffraction_limit dbr:Microscopy dbr:Electron_tomography dbr:Histological_section dbr:Deconvolution dbr:X-ray_microscope dbr:Aperture dbr:Scanning_probe_microscope dbr:Magnification dbr:Concentration dbr:Tomography dbr:Shallow_focus dbr:Photography dbr:Thin_section dbr:Structured_illumination_microscopy dbr:Optical_resolution dbr:Electron_microscope dbr:Microscope dbr:Energy dbr:Light_microscope dbc:Microscopy dbr:Focal_plane dbr:Interferometry dbr:Fluorescence_microscopy dbr:Depth_of_field dbr:Photon dbr:Numerical_aperture dbr:Charge-coupled_device dbr:Total_internal_reflection_microscopy dbr:Conjugate_focal_plane dbr:Refraction
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dbo:abstract
Optical sectioning is the process by which a suitably designed microscope can produce clear images of focal planes deep within a thick sample. This is used to reduce the need for thin sectioning using instruments such as the microtome. Many different techniques for optical sectioning are used and several microscopy techniques are specifically designed to improve the quality of optical sectioning. Good optical sectioning, often referred to as good depth or z resolution, is popular in modern microscopy as it allows the three-dimensional reconstruction of a sample from images captured at different focal planes.
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