. . "36991"^^ . "Cre-loxP\u90E8\u4F4D\u7279\u7570\u7684\u7D44\u63DB\u3048\u306F\u30011981\u5E74\u306B\u30D0\u30AF\u30C6\u30EA\u30AA\u30D5\u30A1\u30FC\u30B8P1\u306E\u7814\u7A76\u3067\u898B\u51FA\u3055\u308C\u305F\u53CD\u5FDC\u3067\u3042\u308B\u3002loxP\u3068\u3044\u3046\u7279\u5B9A\u306EDNA\u914D\u5217\u3092\u6A19\u7684\u3068\u3057\u3066\u304A\u308A\u3001DNA\u7D44\u63DB\u3048\u9175\u7D20Cre\u306B\u3088\u308A\u89E6\u5A92\u3055\u308C\u308B\u30021987\u5E74\u30C7\u30E5\u30DD\u30F3\u793E\uFF08\u5F53\u6642\uFF09\u306EBrian Sauer\u304C\u771F\u6838\u751F\u7269\u3067\u306E\u5FDC\u7528\u6CD5\u3092\u958B\u767A\u3057\u305F\u306E\u3092\u7AEF\u7DD2\u306B\u3001\u73FE\u5728\u3067\u306F\u6761\u4EF6\u7684\u907A\u4F1D\u5B50\u30CE\u30C3\u30AF\u30A2\u30A6\u30C8\u3092\u5B9F\u65BD\u3059\u308B\u76EE\u7684\u306A\u3069\u3067\u5E83\u304F\u4F7F\u308F\u308C\u308B\u6280\u8853\u3068\u306A\u3063\u3066\u3044\u308B\u3002"@ja . . . . . . . . . "loxP-Cre rekombineringssystemet \u00E4r en molekyl\u00E4rgenetisk metod f\u00F6r att unders\u00F6ka effekten av olika gener i specifika v\u00E4vnader. Metoden anv\u00E4nds f\u00F6r att skapa en s\u00E5 kallad knockoutmus, eller annan knockoutorganism, d\u00E4r en viss gen i en viss celltyp st\u00E4ngs av. loxP \u00E4r speciella rekombineringssiter p\u00E5 DNA, en form av repeterade sekvenser som kan omge ett exon. Cre \u00E4r en gen som kodar f\u00F6r ett protein som binder till IoxP-sekvenser p\u00E5 DNA-str\u00E4ngen, och inducerar homolog \u00F6verkorsning \u00F6ver de tv\u00E5 IoxP-fragmenten s\u00E5 att DNA-materialet mellan loxP-fragmenten spj\u00E4lkas bort och bildar en liten plasmid."@sv . . . . "LoxP-Cre rekombineringssystem"@sv . . "loxP-Cre rekombineringssystemet \u00E4r en molekyl\u00E4rgenetisk metod f\u00F6r att unders\u00F6ka effekten av olika gener i specifika v\u00E4vnader. Metoden anv\u00E4nds f\u00F6r att skapa en s\u00E5 kallad knockoutmus, eller annan knockoutorganism, d\u00E4r en viss gen i en viss celltyp st\u00E4ngs av. loxP \u00E4r speciella rekombineringssiter p\u00E5 DNA, en form av repeterade sekvenser som kan omge ett exon. Cre \u00E4r en gen som kodar f\u00F6r ett protein som binder till IoxP-sekvenser p\u00E5 DNA-str\u00E4ngen, och inducerar homolog \u00F6verkorsning \u00F6ver de tv\u00E5 IoxP-fragmenten s\u00E5 att DNA-materialet mellan loxP-fragmenten spj\u00E4lkas bort och bildar en liten plasmid. Rent praktiskt g\u00E5r metoden ut p\u00E5 att via homolog rekombination skapa m\u00F6ss som b\u00E5de har loxP-sekvenser som omger genen man vill st\u00E4nga av, samt som har en gen f\u00F6r Cre som styrs av en celltyps-specifik promotor. N\u00E4r cellen differentierats till en celltyp som producerar aktivatorer som aktiverar promotorn f\u00F6r Cre-genen s\u00E5 kommer Cre-protein att bildas, som kommer att spj\u00E4lka bort och d\u00E4rmed f\u00F6rst\u00F6ra genen som omges av loxP-sekvens."@sv . "Das Cre/loxP-System ist ein Rekombinations-System. Es erm\u00F6glicht das gezielte Entfernen von DNA-Sequenzen in lebenden Organismen. Mit dieser Technik k\u00F6nnen beispielsweise einzelne spezifische Zell- oder Gewebearten genetisch modifiziert werden, w\u00E4hrend andere Gewebe davon unber\u00FChrt bleiben. Urspr\u00FCnglich stammt dieses System aus dem Bakteriophagen P1 und findet heute v. a. in der molekularbiologischen Forschung und zur Herstellung gentechnisch ver\u00E4nderter Pflanzen Anwendung. Ein analoges System ist das Flp-FRT-System."@de . . . "Das Cre/loxP-System ist ein Rekombinations-System. Es erm\u00F6glicht das gezielte Entfernen von DNA-Sequenzen in lebenden Organismen. Mit dieser Technik k\u00F6nnen beispielsweise einzelne spezifische Zell- oder Gewebearten genetisch modifiziert werden, w\u00E4hrend andere Gewebe davon unber\u00FChrt bleiben. Urspr\u00FCnglich stammt dieses System aus dem Bakteriophagen P1 und findet heute v. a. in der molekularbiologischen Forschung und zur Herstellung gentechnisch ver\u00E4nderter Pflanzen Anwendung. Ein analoges System ist das Flp-FRT-System."@de . . "Cre-loxP\u90E8\u4F4D\u7279\u7570\u7684\u7D44\u63DB\u3048\u306F\u30011981\u5E74\u306B\u30D0\u30AF\u30C6\u30EA\u30AA\u30D5\u30A1\u30FC\u30B8P1\u306E\u7814\u7A76\u3067\u898B\u51FA\u3055\u308C\u305F\u53CD\u5FDC\u3067\u3042\u308B\u3002loxP\u3068\u3044\u3046\u7279\u5B9A\u306EDNA\u914D\u5217\u3092\u6A19\u7684\u3068\u3057\u3066\u304A\u308A\u3001DNA\u7D44\u63DB\u3048\u9175\u7D20Cre\u306B\u3088\u308A\u89E6\u5A92\u3055\u308C\u308B\u30021987\u5E74\u30C7\u30E5\u30DD\u30F3\u793E\uFF08\u5F53\u6642\uFF09\u306EBrian Sauer\u304C\u771F\u6838\u751F\u7269\u3067\u306E\u5FDC\u7528\u6CD5\u3092\u958B\u767A\u3057\u305F\u306E\u3092\u7AEF\u7DD2\u306B\u3001\u73FE\u5728\u3067\u306F\u6761\u4EF6\u7684\u907A\u4F1D\u5B50\u30CE\u30C3\u30AF\u30A2\u30A6\u30C8\u3092\u5B9F\u65BD\u3059\u308B\u76EE\u7684\u306A\u3069\u3067\u5E83\u304F\u4F7F\u308F\u308C\u308B\u6280\u8853\u3068\u306A\u3063\u3066\u3044\u308B\u3002"@ja . "Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems. The Cre-lox recombination system has been particularly useful to help neuroscientists to study the brain in which complex cell types and neural circuits come together to generate cognition and behaviors. NIH Blueprint for Neuroscience Research has created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community."@en . . "1093612362"^^ . . . . . . . . . "Cre-Lox\u91CD\u7EC4\u662F\u4E00\u79CD\u7528\u4E8E\u5728\u7EC6\u80DEDNA\u7684\u7279\u5B9A\u4F4D\u70B9\u4E0A\u8FDB\u884C\u5220\u9664\u3001\u63D2\u5165\u3001\u8F6C\u5EA7\u548C\u64CD\u4F5C\u7684\uFF0C\u5176\u7279\u70B9\u5728\u4E8E\u53EF\u4EE5\u8BA9DNA\u4FEE\u6539\u5BF9\u6307\u5B9A\u7684\u7279\u5B9A\u7EC6\u80DE\u7FA4\u8FDB\u884C\uFF0C\u6216\u662F\u4F7F\u4FEE\u6539\u8FC7\u7A0B\u7531\u7279\u5B9A\u7684\u5916\u90E8\u523A\u6FC0\u6240\u89E6\u53D1\u3002\u6B64\u6280\u672F\u5BF9\u771F\u6838\u548C\u539F\u6838\u7EC6\u80DE\u90FD\u9002\u7528\u3002\u7279\u522B\u5730\uFF0C\u5BF9\u7814\u7A76\u7531\u5404\u79CD\u590D\u6742\u7EC6\u80DE\u548C\u795E\u7ECF\u901A\u8DEF\u590D\u5408\u800C\u5F62\u6210\u8BA4\u77E5\u884C\u4E3A\u7684\u5927\u8111\u7684\u800C\u8A00\uFF0C\u8FD9\u79CD\u6280\u672F\u5DF2\u88AB\u8BC1\u660E\u662F\u76F8\u5F53\u5B9E\u7528\u7684\u3002 \u8FD9\u4E2A\u91CD\u7EC4\u4F53\u7CFB\u7531\u5355\u4E2A\u9176\u7EC4\u6210\uFF0C\u5373\u53EF\u4EE5\u8BA9\u4E00\u5BF9\u88AB\u79F0\u4E3ALox\u5E8F\u5217\u7684\u77ED\u76EE\u6807\u5E8F\u5217\u8FDB\u884C\u91CD\u7EC4\u7684\uFF0C\u9664\u6B64\u4E4B\u5916\u4E0D\u9700\u8981\u63D2\u5165\u5176\u4ED6\u7684\u989D\u5916\u86CB\u767D\u6216\u5E8F\u5217\u3002Cre\u91CD\u7EC4\u9176\u548C\u6E90Lox\u5E8F\u5217\uFF08\u5373LoxP\u5E8F\u5217\uFF09\u662F\u4ECEP1\u566C\u83CC\u4F53\u4E2D\u83B7\u53D6\u7684\u3002 \u901A\u8FC7\u5408\u9002\u5730\u63D2\u5165Lox\u5E8F\u5217\u53EF\u4EE5\u4F7F\u7279\u5B9A\u57FA\u56E0\u6D3B\u5316\u3001\u6C89\u9ED8\u6216\u8005\u548C\u5176\u4ED6\u57FA\u56E0\u4EA4\u6362\u4F4D\u7F6E\u2014\u2014\u5728DNA\u7684\u5C42\u9762\u4E0A\uFF0C\u8BB8\u591A\u64CD\u4F5C\u90FD\u53EF\u4EE5\u8FDB\u884C\u3002Cre\u9176\u7684\u6D3B\u6027\u53EF\u63A7\uFF0C\u53EF\u4EE5\u4F7F\u4E4B\u53EA\u5728\u4E00\u79CD\u7279\u5B9A\u7EC6\u80DE\u7C7B\u578B\u4E2D\u8868\u8FBE\uFF0C\u4E5F\u53EF\u4EE5\u4F7F\u4E4B\u7531\u7279\u5B9A\u7684\u5916\u90E8\u523A\u6FC0\uFF08\u5982\u5316\u5B66\u4FE1\u53F7\u3001\u70ED\u523A\u6FC0\uFF09\u89E6\u53D1\u8868\u8FBE\u3002\u8FD9\u4E9B\u7279\u5F02\u6027DNA\u4FEE\u9970\u5BF9\u7EC6\u80DE\u7CFB\u8FFD\u8E2A\u4EE5\u53CA\u6709\u81F4\u547D\u6027\u7684\u7A81\u53D8\u7814\u7A76\u5341\u5206\u6709\u6548\u3002 Cre-Lox\u91CD\u7EC4\u7CFB\u7EDF\u5728\u64CD\u4F5C\u548C\u7528\u9014\u4E0A\u90FD\u4E0E\u7CFB\u7EDF\u5341\u5206\u76F8\u4F3C\u3002"@zh . "Cre-Lox\u91CD\u7EC4\u662F\u4E00\u79CD\u7528\u4E8E\u5728\u7EC6\u80DEDNA\u7684\u7279\u5B9A\u4F4D\u70B9\u4E0A\u8FDB\u884C\u5220\u9664\u3001\u63D2\u5165\u3001\u8F6C\u5EA7\u548C\u64CD\u4F5C\u7684\uFF0C\u5176\u7279\u70B9\u5728\u4E8E\u53EF\u4EE5\u8BA9DNA\u4FEE\u6539\u5BF9\u6307\u5B9A\u7684\u7279\u5B9A\u7EC6\u80DE\u7FA4\u8FDB\u884C\uFF0C\u6216\u662F\u4F7F\u4FEE\u6539\u8FC7\u7A0B\u7531\u7279\u5B9A\u7684\u5916\u90E8\u523A\u6FC0\u6240\u89E6\u53D1\u3002\u6B64\u6280\u672F\u5BF9\u771F\u6838\u548C\u539F\u6838\u7EC6\u80DE\u90FD\u9002\u7528\u3002\u7279\u522B\u5730\uFF0C\u5BF9\u7814\u7A76\u7531\u5404\u79CD\u590D\u6742\u7EC6\u80DE\u548C\u795E\u7ECF\u901A\u8DEF\u590D\u5408\u800C\u5F62\u6210\u8BA4\u77E5\u884C\u4E3A\u7684\u5927\u8111\u7684\u800C\u8A00\uFF0C\u8FD9\u79CD\u6280\u672F\u5DF2\u88AB\u8BC1\u660E\u662F\u76F8\u5F53\u5B9E\u7528\u7684\u3002 \u8FD9\u4E2A\u91CD\u7EC4\u4F53\u7CFB\u7531\u5355\u4E2A\u9176\u7EC4\u6210\uFF0C\u5373\u53EF\u4EE5\u8BA9\u4E00\u5BF9\u88AB\u79F0\u4E3ALox\u5E8F\u5217\u7684\u77ED\u76EE\u6807\u5E8F\u5217\u8FDB\u884C\u91CD\u7EC4\u7684\uFF0C\u9664\u6B64\u4E4B\u5916\u4E0D\u9700\u8981\u63D2\u5165\u5176\u4ED6\u7684\u989D\u5916\u86CB\u767D\u6216\u5E8F\u5217\u3002Cre\u91CD\u7EC4\u9176\u548C\u6E90Lox\u5E8F\u5217\uFF08\u5373LoxP\u5E8F\u5217\uFF09\u662F\u4ECEP1\u566C\u83CC\u4F53\u4E2D\u83B7\u53D6\u7684\u3002 \u901A\u8FC7\u5408\u9002\u5730\u63D2\u5165Lox\u5E8F\u5217\u53EF\u4EE5\u4F7F\u7279\u5B9A\u57FA\u56E0\u6D3B\u5316\u3001\u6C89\u9ED8\u6216\u8005\u548C\u5176\u4ED6\u57FA\u56E0\u4EA4\u6362\u4F4D\u7F6E\u2014\u2014\u5728DNA\u7684\u5C42\u9762\u4E0A\uFF0C\u8BB8\u591A\u64CD\u4F5C\u90FD\u53EF\u4EE5\u8FDB\u884C\u3002Cre\u9176\u7684\u6D3B\u6027\u53EF\u63A7\uFF0C\u53EF\u4EE5\u4F7F\u4E4B\u53EA\u5728\u4E00\u79CD\u7279\u5B9A\u7EC6\u80DE\u7C7B\u578B\u4E2D\u8868\u8FBE\uFF0C\u4E5F\u53EF\u4EE5\u4F7F\u4E4B\u7531\u7279\u5B9A\u7684\u5916\u90E8\u523A\u6FC0\uFF08\u5982\u5316\u5B66\u4FE1\u53F7\u3001\u70ED\u523A\u6FC0\uFF09\u89E6\u53D1\u8868\u8FBE\u3002\u8FD9\u4E9B\u7279\u5F02\u6027DNA\u4FEE\u9970\u5BF9\u7EC6\u80DE\u7CFB\u8FFD\u8E2A\u4EE5\u53CA\u6709\u81F4\u547D\u6027\u7684\u7A81\u53D8\u7814\u7A76\u5341\u5206\u6709\u6548\u3002 Cre-Lox\u91CD\u7EC4\u7CFB\u7EDF\u5728\u64CD\u4F5C\u548C\u7528\u9014\u4E0A\u90FD\u4E0E\u7CFB\u7EDF\u5341\u5206\u76F8\u4F3C\u3002"@zh . . . . . "Cre-Lox\u91CD\u7EC4"@zh . "Cre-Lox recombination is a site-specific recombinase technology, used to carry out deletions, insertions, translocations and inversions at specific sites in the DNA of cells. It allows the DNA modification to be targeted to a specific cell type or be triggered by a specific external stimulus. It is implemented both in eukaryotic and prokaryotic systems. The Cre-lox recombination system has been particularly useful to help neuroscientists to study the brain in which complex cell types and neural circuits come together to generate cognition and behaviors. NIH Blueprint for Neuroscience Research has created several hundreds of Cre driver mouse lines which are currently used by the worldwide neuroscience community. The system consists of a single enzyme, Cre recombinase, that recombines a pair of short target sequences called the Lox sequences. This system can be implemented without inserting any extra supporting proteins or sequences. The Cre enzyme and the original Lox site called the LoxP sequence are derived from bacteriophage P1. Placing Lox sequences appropriately allows genes to be activated, repressed, or exchanged for other genes. At a DNA level many types of manipulations can be carried out. The activity of the Cre enzyme can be controlled so that it is expressed in a particular cell type or triggered by an external stimulus like a chemical signal or a heat shock. These targeted DNA changes are useful in cell lineage tracing and when mutants are lethal if expressed globally. The Cre-Lox system is very similar in action and in usage to the FLP-FRT recombination system."@en . . . "Recombina\u00E7\u00E3o Cre-Lox"@pt . . . . . . . . . . . . "Cre-Lox recombination"@en . "4363576"^^ . . . . . . . . . "Cre/loxP-System"@de . . "Recombina\u00E7\u00E3o Cre-Lox \u00E9 uma tecnologia de recombina\u00E7\u00E3o s\u00EDtio-espec\u00EDfica, cujo nome vem do uso da enzima e . Derivada do mecanismo natural de recombina\u00E7\u00E3o do , a recombina\u00E7\u00E3o Cre-Lox \u00E9 utilizada para diversos usos cient\u00EDficos e tecnol\u00F3gicos, notavelmente inser\u00E7\u00F5es, dele\u00E7\u00F5es, invers\u00F5es e transloca\u00E7\u00F5es s\u00EDtio-espec\u00EDficas em sequ\u00EAncias de DNA. Diz-se que uma sequ\u00EAncia \u00E9 \u201Cfloxed\u201D quando est\u00E1 flanqueada por s\u00EDtios Lox. Um dos usos mais conhecidos da recombina\u00E7\u00E3o Cre-Lox \u00E9 a cria\u00E7\u00E3o de animais knockout condicionais, onde a modifica\u00E7\u00E3o desejada ocorrer\u00E1 apenas ap\u00F3s um est\u00EDmulo desejado. Isso permite a altera\u00E7\u00E3o de sequ\u00EAncias de DNA em tecidos espec\u00EDficos ou em momentos espec\u00EDficos do desenvolvimento, driblando poss\u00EDveis efeitos letais se a altera\u00E7\u00E3o ocorresse globalmente."@pt . "Cre-loxP\u90E8\u4F4D\u7279\u7570\u7684\u7D44\u63DB\u3048"@ja . . "Recombina\u00E7\u00E3o Cre-Lox \u00E9 uma tecnologia de recombina\u00E7\u00E3o s\u00EDtio-espec\u00EDfica, cujo nome vem do uso da enzima e . Derivada do mecanismo natural de recombina\u00E7\u00E3o do , a recombina\u00E7\u00E3o Cre-Lox \u00E9 utilizada para diversos usos cient\u00EDficos e tecnol\u00F3gicos, notavelmente inser\u00E7\u00F5es, dele\u00E7\u00F5es, invers\u00F5es e transloca\u00E7\u00F5es s\u00EDtio-espec\u00EDficas em sequ\u00EAncias de DNA. Diz-se que uma sequ\u00EAncia \u00E9 \u201Cfloxed\u201D quando est\u00E1 flanqueada por s\u00EDtios Lox."@pt . . . . . . . . . . . . .