The 2021 NCAA Division I softball tournament was held from May 21 through June 10, 2021 as the final part of the 2021 NCAA Division I softball season. 31 teams were awarded automatic bids as champions of their conferences after the Ivy League opted out of the 2021 softball season. The remaining 33 were selected at-large by the NCAA Division I softball selection committee on May 16, 2021. The tournament culminated with eight teams playing in the 2021 Women's College World Series at USA Softball Hall of Fame Stadium in Oklahoma City.