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In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. In the old days, this was accomplished by the Edman degradation procedure. Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. Generally, there are two approaches: database search and de novo sequencing. Database search is a simple version as the mass spectra data of the unknown peptide is submitted and run to find a match with a known peptide sequence, the peptide with the highest matching score will be selected. This approach fails to recognize novel peptides

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  • De-Novo-Peptidsequenzierung (de)
  • De novo peptide sequencing (en)
  • 신해독 (ko)
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  • Die De-Novo-Peptidsequenzierung ist ein biochemisches Verfahren zur Bestimmung der Aminosäuresequenz von Proteinen oder Peptiden per Tandem-Massenspektrometrie (MS/MS). Sie ist eine Methode zur Proteinsequenzierung. (de)
  • 신해독은 게놈학(유전체학) 분야에서 쓰이는 용어로, de novo sequencing 을 번역한 말이다. 게놈을 해독하는 방법은 크게 두가지로 분류될수 있다. 하나는 re-sequencing (재해독)이고, 다른 하나는 de novo sequencing (신해독 혹은 선도해독)이다. 이것은 게놈지도를 만들때 기본적으로 그 목적과 개념이 다르다. 총 4조원이 든 최초의 인간 표준게놈은 신해독 방법으로 게놈지도를 새로 만든것이다. 2016년도, 천달러인 개개인의 재해독은 완전히 다른 비용계산이 필요하다. 이것은 신해독된 표준게놈에, 해독된 주로 짧은 서열을 정렬을 시켜서 그 뼈대에 맞춰 구성한 일종의 반쪽자리 게놈지도이다. (ko)
  • In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. In the old days, this was accomplished by the Edman degradation procedure. Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. Generally, there are two approaches: database search and de novo sequencing. Database search is a simple version as the mass spectra data of the unknown peptide is submitted and run to find a match with a known peptide sequence, the peptide with the highest matching score will be selected. This approach fails to recognize novel peptides (en)
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  • http://commons.wikimedia.org/wiki/Special:FilePath/6_sequence_ions.png
  • http://commons.wikimedia.org/wiki/Special:FilePath/Amino_acid_fragment_ions.png
  • http://commons.wikimedia.org/wiki/Special:FilePath/Masses_of_b2-ions.png
  • http://commons.wikimedia.org/wiki/Special:FilePath/Satellite_ions.png
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  • Die De-Novo-Peptidsequenzierung ist ein biochemisches Verfahren zur Bestimmung der Aminosäuresequenz von Proteinen oder Peptiden per Tandem-Massenspektrometrie (MS/MS). Sie ist eine Methode zur Proteinsequenzierung. (de)
  • In mass spectrometry, de novo peptide sequencing is the method in which a peptide amino acid sequence is determined from tandem mass spectrometry. Knowing the amino acid sequence of peptides from a protein digest is essential for studying the biological function of the protein. In the old days, this was accomplished by the Edman degradation procedure. Today, analysis by a tandem mass spectrometer is a more common method to solve the sequencing of peptides. Generally, there are two approaches: database search and de novo sequencing. Database search is a simple version as the mass spectra data of the unknown peptide is submitted and run to find a match with a known peptide sequence, the peptide with the highest matching score will be selected. This approach fails to recognize novel peptides since it can only match to existing sequences in the database. De novo sequencing is an assignment of fragment ions from a mass spectrum. Different algorithmsare used for interpretation and most instruments come with de novo sequencing programs. (en)
  • 신해독은 게놈학(유전체학) 분야에서 쓰이는 용어로, de novo sequencing 을 번역한 말이다. 게놈을 해독하는 방법은 크게 두가지로 분류될수 있다. 하나는 re-sequencing (재해독)이고, 다른 하나는 de novo sequencing (신해독 혹은 선도해독)이다. 이것은 게놈지도를 만들때 기본적으로 그 목적과 개념이 다르다. 총 4조원이 든 최초의 인간 표준게놈은 신해독 방법으로 게놈지도를 새로 만든것이다. 2016년도, 천달러인 개개인의 재해독은 완전히 다른 비용계산이 필요하다. 이것은 신해독된 표준게놈에, 해독된 주로 짧은 서열을 정렬을 시켜서 그 뼈대에 맞춰 구성한 일종의 반쪽자리 게놈지도이다. (ko)
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