Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation of selected ions in the gas phase. The selected ions (typically molecular ions or protonated molecules) are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules (often helium, nitrogen or argon). In the collision some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry.
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| - Dissociació induïda per col·lisió (ca)
- Kollisionsinduzierte Dissoziation (de)
- Collision-induced dissociation (en)
- Dissociazione indotta da collisione (it)
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| - Die kollisionsinduzierte Dissoziation (englisch collision-induced dissociation, CID, synonym collisionally activated dissociation) ist eine Methode zur Fragmentierung von Molekülionen in der Gasphase bei der Massenspektrometrie. (de)
- In spettrometria di massa tandem, la dissociazione indotta da collisione (collision-induced dissociation, CID in inglese) o dissociazione attivata da collisione (collision-activated dissociation, CAD in inglese), è un meccanismo di frammentazione degli ioni in fase gas. Gli ioni sono generalmente accelerati nel vuoto da un potenziale elettrico e fatti collidere con molecole neutre in fase gas (solitamente elio, azoto o argon). Nella collisione una parte dell'energia cinetica è convertita in energia interna e ciò fa sì che si rompano uno o più legami e lo ione sia frammentato. I frammenti sono inviati in uno spettrometro di massa. (it)
- La Dissociació induïda per col·lisió (en anglès, Collision-induced dissociation o CID, o Collisionally activated dissociation o CAD), és una tècnica d'espectrometria de masses per induir la fragmentació de molècules ionitzades en fase gas. La tècnica de dissociació induïda per col·lisió és utilitzada per diversos propòsits entre els quals cal destacar la determinació estructural i la seqüenciació de peptídica. (ca)
- Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation of selected ions in the gas phase. The selected ions (typically molecular ions or protonated molecules) are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules (often helium, nitrogen or argon). In the collision some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry. (en)
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| - La Dissociació induïda per col·lisió (en anglès, Collision-induced dissociation o CID, o Collisionally activated dissociation o CAD), és una tècnica d'espectrometria de masses per induir la fragmentació de molècules ionitzades en fase gas. En aquest mode de fragmentació els ions són accelerats, normalment per un potencial elèctric que els confereix una elevada energia cinètica, i a continuació, se'ls fa col·lidir amb molècules neutres (sovint heli, nitrogen o argó). Durant la col·lisió part de l'energia cinètica es converteix en energia interna que sovint dona lloc al trencament d'un enllaç molecular i, conseqüentment, a la fragmentació de la molècula en fragments més petits. Aquests fragments llavors es poden analitzar mitjançant un . La tècnica de dissociació induïda per col·lisió és utilitzada per diversos propòsits entre els quals cal destacar la determinació estructural i la seqüenciació de peptídica. (ca)
- Die kollisionsinduzierte Dissoziation (englisch collision-induced dissociation, CID, synonym collisionally activated dissociation) ist eine Methode zur Fragmentierung von Molekülionen in der Gasphase bei der Massenspektrometrie. (de)
- Collision-induced dissociation (CID), also known as collisionally activated dissociation (CAD), is a mass spectrometry technique to induce fragmentation of selected ions in the gas phase. The selected ions (typically molecular ions or protonated molecules) are usually accelerated by applying an electrical potential to increase the ion kinetic energy and then allowed to collide with neutral molecules (often helium, nitrogen or argon). In the collision some of the kinetic energy is converted into internal energy which results in bond breakage and the fragmentation of the molecular ion into smaller fragments. These fragment ions can then be analyzed by tandem mass spectrometry. CID and the fragment ions produced by CID are used for several purposes. Partial or complete structural determination can be achieved. In some cases identity can be established based on previous knowledge without determining structure. Another use is in simply achieving more sensitive and specific detection. By detecting a unique fragment ion, the precursor ion can be detected in the presence of other ions of the same m/z value (mass-to-charge ratio), reducing the background and increasing the limit of detection. (en)
- In spettrometria di massa tandem, la dissociazione indotta da collisione (collision-induced dissociation, CID in inglese) o dissociazione attivata da collisione (collision-activated dissociation, CAD in inglese), è un meccanismo di frammentazione degli ioni in fase gas. Gli ioni sono generalmente accelerati nel vuoto da un potenziale elettrico e fatti collidere con molecole neutre in fase gas (solitamente elio, azoto o argon). Nella collisione una parte dell'energia cinetica è convertita in energia interna e ciò fa sì che si rompano uno o più legami e lo ione sia frammentato. I frammenti sono inviati in uno spettrometro di massa. (it)
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