John Hartley Chamberlain (29 April 1884 – 16 January 1953) was an Australian politician. He served as a Senator for Tasmania from 1951 until his death in 1953, representing the Liberal Party. He was also a member of the Tasmanian House of Assembly from 1934 to 1951, briefly serving as deputy opposition leader. He was a farmer at Latrobe prior to entering politics, having taken up land as a soldier settler.