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In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic acids (such as DNA or RNA) in a solution. These are spectrophotometric quantification and UV fluorescence tagging in presence of a DNA dye.

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  • Cuantificación de ácidos nucleicos (es)
  • Nucleic acid quantitation (en)
  • Количественный анализ нуклеиновых кислот (ru)
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  • En biología molecular, la cuantificación de ácidos nucleicos se realiza habitualmente para determinar las concentraciones medias de ADN o ARN presentes en una mezcla, así como su pureza. Las reacciones en las que se utilizan ácidos nucleicos suelen requerir cantidades y pureza particulares para un rendimiento óptimo. Hasta la fecha, existen dos enfoques principales utilizados por los científicos para cuantificar, o establecer la concentración, de ácidos nucleicos (como el ADN o el ARN) en una solución. Se trata de la cuantificación espectrofotométrica y el marcado por fluorescencia UV en presencia de un colorante de ADN.​ (es)
  • In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic acids (such as DNA or RNA) in a solution. These are spectrophotometric quantification and UV fluorescence tagging in presence of a DNA dye. (en)
  • Количественный анализ нуклеиновых кислот — определение концентрации ДНК или РНК в смеси или чистом препарате. Реакции с участием нуклеиновых кислот часто требуют точных сведений о количестве и чистоте препарата. Для определения концентрации нуклеиновой кислоты в растворе используют спектрофотометрический метод и УФ-флюоресценцию, если нуклеиновая кислота содержит краситель. (ru)
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  • En biología molecular, la cuantificación de ácidos nucleicos se realiza habitualmente para determinar las concentraciones medias de ADN o ARN presentes en una mezcla, así como su pureza. Las reacciones en las que se utilizan ácidos nucleicos suelen requerir cantidades y pureza particulares para un rendimiento óptimo. Hasta la fecha, existen dos enfoques principales utilizados por los científicos para cuantificar, o establecer la concentración, de ácidos nucleicos (como el ADN o el ARN) en una solución. Se trata de la cuantificación espectrofotométrica y el marcado por fluorescencia UV en presencia de un colorante de ADN.​ (es)
  • In molecular biology, quantitation of nucleic acids is commonly performed to determine the average concentrations of DNA or RNA present in a mixture, as well as their purity. Reactions that use nucleic acids often require particular amounts and purity for optimum performance. To date, there are two main approaches used by scientists to quantitate, or establish the concentration, of nucleic acids (such as DNA or RNA) in a solution. These are spectrophotometric quantification and UV fluorescence tagging in presence of a DNA dye. (en)
  • Количественный анализ нуклеиновых кислот — определение концентрации ДНК или РНК в смеси или чистом препарате. Реакции с участием нуклеиновых кислот часто требуют точных сведений о количестве и чистоте препарата. Для определения концентрации нуклеиновой кислоты в растворе используют спектрофотометрический метод и УФ-флюоресценцию, если нуклеиновая кислота содержит краситель. (ru)
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