Penmanshiel Tunnel is a now-disused railway tunnel near Grantshouse, Berwickshire, in the Scottish Borders region of Scotland. It was formerly part of the East Coast Main Line between Berwick-upon-Tweed and Dunbar. The tunnel was constructed during 1845–46 by the contractors Ross and Mitchell, to a design by John Miller, who was the Engineer to the North British Railway. Upon completion, the tunnel was inspected by the Inspector-General of Railways, Major-General Charles Pasley, on behalf of the Board of Trade.