Pennsylvania Route 316 (PA 316) is a 16.6-mile-long (26.7 km) state highway located in Franklin County, Pennsylvania. The southern terminus is at the Maryland state line near Waynesboro, where the road continues into that state as Maryland Route 60 (MD 60). The northern terminus is at U.S. Route 11 (US 11) in Chambersburg. PA 316 heads north from the state line through rural land to Waynesboro, where it forms a concurrency with PA 16. From here, the route continues through farmland, intersecting PA 914 south of New Franklin. PA 316 reaches Chambersburg and runs through developed area, coming to an interchange with Interstate 81 (I-81) before ending at US 11.