St John's Cemetery, Parramatta, also known as St John's Anglican Cemetery, Saint John's Cemetery, and First Fleet Cemetery, is a heritage-listed cemetery at 1 O'Connell Street, Parramatta, City of Parramatta, New South Wales, Australia. It was only when cemeteries dedicated to specific religious denominations were progressively established in Parramatta in the mid-nineteenth century that St John's finally became an Anglican burial ground specifically. The historic cemetery was added to the New South Wales State Heritage Register on 2 April 1999.