Tunku Naquiyuddin ibni Almarhum Tuanku Ja'afar is the eldest son of Tuanku Jaafar ibni Almarhum Tuanku Abdul Rahman, the Yang di-Pertuan Besar of Negeri Sembilan, who was also a former Yang di-Pertuan Agong (King) of Malaysia. He served as regent of Negeri Sembilan from 1994-1999 when his father was elected as the King of Malaysia.