The 2019–20 SFA South Region Challenge Cup was the 13th edition of the annual cup competition for senior non-league clubs in the central and southern regions of Scotland. This season saw the competition increase to 70 teams despite the demise of Selkirk and withdrawal of Eyemouth United, thanks to the relegation of Berwick Rangers to the Lowland League plus the addition of Glenrothes and Kinnoull to the East of Scotland Football League.